Please note
This document only provides information for the academic year selected and does not form part of the student contract
School:
School of Applied Sciences
Credit Rating:
20
Level (including FHEQ):
I (FHEQ Level 5)
Graded or Non Graded:
Graded
Version Valid From:
2019-09-02
Module Leader:
Hazel Girvan
Version Number
2023.03
Learning Methods
Seminar
Guided Independent Study
Lecture
Practical Classes and Demonstrations
Requirements
Pre-requisites
Recommended Prior Study
Synopsis
This module considers protein structure in relation to function, in particular enzyme action and membrane mediated metabolic processes.
The various types of post-translational modifications of proteins are discussed in detail, followed by an account of protein folding. Protein mis-folding, aggregation and intrinsic disorder are discussed using prion proteins, amyloid and … For more content click the Read More button below.
The techniques of X-ray crystallography and Cryo-EM for protein structure determination are introduced. Advantages and limitations are discussed.
Lectures discuss various techniques for analyzing protein structure, folding and protein-protein interactions (isothermal titration calorimetry, near and far UV circular dichroism)
The structure and function of mitochondria and chloroplasts is covered, including respiration and photosynthesis pathways, focusing on electron transport chains.
Mechanisms of enzyme catalysis are discussed with numerous examples (acid-base, covalent, nucleophilic, metal ion etc.).
In lectures, practicals and problem based tutorials the skills required for determining kinetic and inhibition constants (kcat, catalytic efficiency, Ki) will be developed.
Practicals also allow the student to isolate a pure protein from egg white, assess product quality and consider the commercial value of this purified material.
Learning Strategy
By a mixture of formal lectures, problem solving tutorials and practical experience of biochemistry it is intended that the student will have an appreciation of the role of proteins as structural, functional and catalytic components of living things.
Outline Syllabus
Techniques for purification, molecular weight determination, sequencing of proteins.Different levels of structure found in fibrous and globular proteins and the forces which stabilize them.Prediction and experimental analysis of secondary structure.X-ray crystallography for protein structure determination.Protein folding and higher levels of organisation of proteins.Outline of photosynthesis, photon capture, and synthesis of … For more content click the Read More button below.
Formative Assessment
Assessment 1: Practice exam/test
Summative Assessment
Assessment 1: Exam
Assessment 2: Written Assignment
Assessment Criteria
Practical competency will be assessed in class by teaching staff.
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